Immunoprecipitation (IP) is a widely used method for neoantigen screening. However, this approach has technical limitations such as antibody dependence/bias, cost, time-consuming and inapplicability in non-human models due to lack of effective antibodies.
The invention includes the application of a benign acid elution method to salivary gland patient tissues for the screening and quantification of neoantigens. This novel approach facilitates the screening of neoantigens directly from patient samples, such as a tumour.
The proposed solution is low-cost, repeatable and fast, making it applicable to many industries.
