Immunoprecipitation (IP) is a widely used method to sreen the neoantigen. However this approach has technical limitationss such as antibody dependant/biased, costly, time-consuming and inability to emploi for other than the human model due to lack of efficient antibodies.
The method has been applied as a mild acid elution approach to salivary gland patient tissues for screening and quantification of neoantigens. The novel approach facilitates the screening of neoantigens directly from patient samples, such as tumours. Moreover, it is a rapid, low-cost, simple, high-throughput, antibody-independent and reproducible approach that can be used worldwide. The tumour approach ultimately opens a wide door for the development and improvement of immunotherapies for humans and animal models, such as dogs, pigs, for many diseases or health problems (cancer, autoimmune diseases, viral and infectious diseases, etc.).
The method has great potential to open up many branches for various diseases, many of which rely on immune response and gown in academic, medical, biotechnological and industrial development.
