The invention provides an efficient method for synthesizing N-maleimidoglycine using alumina-modified silica as a catalyst, followed by its conversion into an active N-hydroxysuccinimide ester and covalent attachment to chitosan under mild conditions.
The solution also includes a quantitative analytical method for determining the degree of substitution via normal-phase HPLC.
The resulting maleimidoglycine-modified chitosan remains biocompatible, enables further chemical functionalization, and serves as a versatile platform for advanced biomedical materials.
Application:
- Chitosan modification for biomedical and material applications,
- Conjugation of proteins, peptides, and nanocarriers via Michael addition,
- Development of functional hydrogels, drug-delivery systems, and bioactive carriers.
